Sunday

Natural High Blood Pressure Cures That Work

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Recetas para salvar el SNS - DiarioMedico.com

Can someone tell me stats about living in Chicago, IL?

Can someone tell me stats about living in Chicago, IL? I would like to know the following: Cost of (per month) -Food -Gas/fuel -Electricity -Telephone -Water -Clothing -Personal care (including haircut, toiletries etc.) INSURANCE -life -health -dental -auto I would really appreiciate it!Thanks in advance.
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It varies by area for a lot of those things.Food is expensive at most chain places (Jewel, Dominick's) usually.Places like Aldi or local grocery stores are cheaper.So is shopping for clothes.If you want to shop by brand that's a little more expensive but places like Filene's basement and TJ Maxx have decent stuff if you're willing to look.Toiletries and what not are pretty much the same anywhere.There's a lot of Walgreens and CVS stores in most places.Gas and water are usually included if you're living in an apartment.I don't know how much they are if you're buying a house.I don't know how insurance is compared to other cities.Hope some of this helps
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Dental Implants Information Dental Implants - A Modern Advancement In Dentistry

This Weekв

This Weekв 
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Parkinsonism. Symptoms, treatment

Parkinsonism. Symptoms, treatment

Clinical presentation includes a triad of symptoms: akinesia, rigidity and tremor of the muscles.Was accompanied by gait disturbance, autonomic dysfunction, and pain.Idiopathic form of the disease usually occurs after age 50 and begins with either shake hands or with partial or total restraint.Gradually, tremor and akinesia generalizes appears stiff muscles, with a gradual increase in tone in the limb during repeated pa*sive movements and tolchkooobraznoy hypertension (cogwheel phenomenon).Changing posture and gait: the patient more stooping, walking with tiny steps, disappear friendly hand movements during walking (aheyrokinez).Oskudevaet mimicry of patients; slowed flashing, his face becomes maskoobraznym.Characterized by the paradoxical kinesis: the patient is not able to walk independently, easily runs up the stairs, can dance, skiing.Some patients have propulsion: the slow movement gives way to sudden rapid run, which the patient can not overcome until the fall or do not meet an obstacle.Observed universality of facial skin, hypersalivation.Often disturbed urination.To a certain extent oskudevaet and emotional life of patients gradually developed dementia.Normal muscle strength, reflex and sensory area does not suffer.The disease usually progresses, but the rate of growth of motor disorders is very different.In severe cases or in late stage disease the patient is almost completely immobilized and can not get out of bed, even with a*sistance.The differential diagnosis of senile tremor based on the absence of the last muscle rigidity.Essential tremor occurs at a younger age.Tremor in thyrotoxicosis and brain fever combined with the characteristic signs of these diseases.Pyramidal spasticity as expressed in the early pa*sive movement (symptom jackknife).Treatment of parkinsonism in the treatment of Parkinson's universal schemes do not.Treatment program for each patient must be individually, however, rationally selected regimen will achieve good results for a long time.The main drugs used in treatment are the means of acting on different neural mediators - acetylcholine, dopamine.These are some groups of antidepressants, antipsychotics, holinolitikov.Previously applied surgical treatment of Parkinson's disease (surgery to destroy parts of the brain responsible for muscle rigidity and tremor), this is practically not used.The reason is quite successful pharmacotherapy, the progress which has in recent years.


{ * InsideFun * } Small Lake Courthouse Towers - Images &amp; Detail

James Franco and Mila Kunis

James Franco and Mila Kunis в

Saturday

Insomnia (Onyx Movie Tie-In)

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Dr. Oz on GMOs III

Dr. Oz on GMOs III Are genetically engineered foods safe?Dr.Oz leads an investigation to find out.See how you can protect your family.Click here for more...
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Sex on the Brain: 12 Lessons to Enhance Your Love Life

Sex on the Brain: 12 Lessons to Enhance Your Love Life

What is your best tool for achieving the ultimate sexual pleasure?Your brain!Everyone wants to know how to improve his or her love life, but so few of us understand the integral role the brain plays in getting us in the mood, keeping us excited about our partner, and helping us achieve greater satisfaction.Based on Dr.Daniel Amen’s cutting-edge research in practical neuroscience, Sex on the Brain shares twelve lessons that help you enhance your love and sex lives through understanding and improving brain function.Filled with practical suggestions and information on how to have better sex, Sex on the Brain reveals: - How the differences between men’s and women’s brains affect our perceptions and interest in sex - The science behind why breakups hurt so much, and what you can do to ease the pain - Surefire techniques to п

House rejects Senate Dems debt-limit bill (AP)

House rejects Senate Dems debt-limit bill  (AP) Republican leaders say fully engaged with Obama (Reuters) Moody's expects to affirm U.S.rating, negative outlook (Reuters) 30 Jul 2011 12:27 PM PDTSpeaker of the House John Boehner, R-Ohio, returns to his office from the House floor as the debt crisis showdown continues on Capitol Hill in Washington, Saturday, July 30, 2011. (AP Photo/J.Scott Applewhite)AP - The Republican-led House on Saturday rejected a Senate Democratic bill to raise the nation's debt limit just three days before the deadline to avert an unprecedented U.S.financial default.President Barack Obama and lawmakers remained at loggerheads on any possible compromise.30 Jul 2011 10:19 AM PDTFILE - In this June 14, 2011, file photo Sen.Jim DeMint, R-S.C., speaks to media on Capitol Hill in Washington. In these nervous days of debt limit warfare and pre-election posturing DeMint, who calls himself Sen.Tea Party, is specific and focused on what change, exactly, he wants: pa*sage Г 
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How to be a courteous hotel guest.

How to be a courteous hotel guest.

I’m weird.We all know this.

But I am obsessed with leaving hotel rooms as clean as I possibly can before checking out.

I realize that it’s not necessarily my job-

that hotels have staff members devoted to that very task.

But I’ve been there-I was once a fresh-faced 18 year old who thought housekeeping in a tourist town for the summer would be a survivable job.

My best friend got hired at the same time.We were ready for some fun.

We were ready to clean rooms across the street from the beauty that is Lake Superior.

Then, reality hit.

It’s a really hard, thankless, physically tiring job.

You’re on your feet all day.You don’t get breaks (at least we didn’t).There is never enough time.

Or towels.There were never, ever enough towels to stock each room.

You’d have to scavenge through housekeeping closets throughout the day, make pit stops in the laundry room, or God forbid, steal some from a fellow housekeeper.

I once made a big mistake while cleaning a room.

I turned the TV on, set the station to MTV, and sat on the bed for a minute or five to rest my feet as I stuffed pillows into their freshly cleaned cases.

I thought that was reasonable enough.My feet ached, and I was still getting something done.

Wrong.В  Fail.I was busted by a housekeeping nutcase who took her job seriously with a capital в

adult clip spanking video

adult clip spanking video What turned me on this strategy. Yes, I thought how cool that this year did not have anyone adult clip spanking video to buy a card for Valentines Day.From time to time I have to run away back home and adult clip spanking video write articles.And then snip-snip, polshtuki bucks, and I went.I chewed all the flakes that were found, adult clip spanking video but the reality is clearly pointed out to me that I am in deep shit.Is there anything in adult clip spanking video life there are no other joys.Just a few minutes ago Jury said that the airport finally opened and adult clip spanking video in Nome too clear.But then, standing in the kitchen next to the Bike-Menom, I did not feel any adult clip spanking video fluids." I felt the bitterness softened, however, that foreseen in advance, as are small chances of success.
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Health Solutions - Stress Relief

Ways To Get Free CNA Training

Ways To Get Free CNA Training There are a number of ways to obtain training for you to become a certified nursing a*sistant, which includes free CNA training.There are in reality quite a few means which you can acquire nursing a*sistant training free of cost.Several long term care facilities, nursing homes and also numerous hospitals offer interested individuals the chance of free CNA training.There are usually certain
[zeezal] Latest Photographs In Beautiful Model Bipasha Basu

Friday

{Jelly Fish}

{Jelly Fish}
Let's just say a big ole' storm brought in a ton of sea creatures & jelly fish was one of them!This morning I looked out the balcony to see that the wh*le shoreline had been moved.All the neighbor condos chairs had been moved closer to the ocean and a big mini ocean pool had been created.Us girls all stayed in the mini pools for the babes & the boys were all playing in the ocean with the nice big waves that we got overnight.2 hours later me, my mom, and chelle were headed back up to the pool from a long walk and noticed this group had caught a jelly fish so I go and look at it & then we were informed by a little girl that there were "millions over there in another bucket..." so I went in and grabbed my camera & came back down with Ty & my mom and seriously, they were pulling jelly fish out like they were seaweed.Ty caught a couple with his little shovel we had for the girls.The kids out there were LOVING it - they all were trying to see how many they could catch.There was like 10 jellyfish coming in with each wave, crazy!!I bet the boys were glad they got out of the ocean when they did!
Corey Taylor takes over Q101.1!

Personal Confession: If you ask me something in person or

Personal Confession: If you ask me something in person or
Personal Confession: If you ask me something in person or online, I will always tell you the straight up truth.I used to always try to make the situation better.But I'm sick of it.I would rather have someone throw me the absolute truth than give me a half a*s answer to make me feel better.

Health Solutions - Stress Relief

Surf's Up Kidside Tropical Smoothie Hair Texturing Wax (2 oz)

Surf's Up Kidside Tropical Smoothie Hair Texturing Wax (2 oz) $10.00Surf's Up Kidside Tropical Smoothie Super Keen Texturing Wax (2 oz). Top the mop with a dollop of Super Keen Hair Texturing Wax for a keen surfer spikes or smooth & shiny style.Non-greasy, exceptionally light formula with excellent hold. Surf's Up Kidside is all about fun! Surf's Up Kidside pure & natural blends are free of Sulfates, Parabens, Phylates, Formaldehyde, DEA, MEA, and TEA. Good for your body and good for the planet. Mahalo. Made in the USA.
Recetas para salvar el SNS - DiarioMedico.com

Quick Noteв

Quick Noteв 
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Gain for High Efficiency Machines Original Scent Powder Detergent 80 Loads, 91-Ounce

Gain for High Efficiency Machines Original Scent Powder Detergent 80 Loads, 91-Ounce Keeps fantastic freshness in your clothes even after two weeks!Original Scent combines these sniff-tastic elements: Overtones: Orange, lemon and blossoms with a modern fruity, green twist.Undertones: White floral jasmine with a hint of woody amber.
Malice intensifies the pain

Cheap Sunbeam SB60 Lint Roller, 60 Sheets

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FREE printable school holiday planner, summer games for kids and teens PLUS which celeb is pregnant?

FREE printable school holiday planner, summer games for kids and teens PLUS which celeb is pregnant? 'Fancy a lie in?I tell the kids they'll get a prize if they stay in bed till the clock says 7.Then while they're asleep, I change the clock back an hour!It's naughty but it works.' How are you feeling after the first week of the summer holidays?We all love spending time with the kids, but it can be exhausting.If they're running out of things to do we've got loads of ideas to keep them out of trouble.Download our free and stick it on the fridge.You'll be surprised how much of a difference it makes to have a plan!Wondering how you're going to keep the kids entertained while they're on school holidays?Print out our FREE week planner, fill it with our ideas of things to do and stick in on your fridge!The old games are the best!Our favourite outdoor kids games Whose tiny hand is this?Find out in Tweeting celeb parents Recetas para salvar el SNS - DiarioMedico.com

Taneytown holding community blood drive - Carroll County Times

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Taneytown holding a*semblage blood drive Carroll part Times Were you alert that one out of every three adults in the United States has high blood pressure , which increases the jeopardy for disease and stroke?The Centers for unwellness Control call high bodily fluid pressure a "silent killer" because it often has no warning ... Link To Article

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Food Informants: A Week In The Life Of Gio Andollo, Freegan

Food Informants: A Week In The Life Of Gio Andollo, Freegan

Food Informants is a week-in-the-life series profiling fascinating people in the food world.We hope it will give you a first-hand look at the many different corners of the food industry.Know someone who would make a great Food Informant?Tell us why.

Freeganism is a lifestyle in which one employs «alternative strategies for living based on limited participation in the conventional economy and minimal consumption of resources.» Gio Andollo is a writer, artist, musician and freegan.Andollo became a freegan when he realized that artists don’t get paid much, but he didn’t like the idea of working a «crappy, part-time job» to pay the bills.So he found another way.Andollo performs on the subway for about 20 hours a week, typically in two-hour intervals.He makes $10 to $50 per shift and has a love/hate relationship with busking.Andollo will buy food, but very rarely.The majority of his food comes from trash touring, or dumpster diving.He’s become increasingly concerned with the abuses inherent in current economic systems: waste of resources, exploitation of people, degradation of the environment, calloused treatment of animals, commodification of time, labor, even war (thus human life in wh*lesale).In addition to busking part-time, he writes songs, blogs and books about these issues.To learn more about Gio, visit his personal blog and portfolio here, his living-on-a-shoestring-in-New-York blog here and his blog about washing dishes here.See a fuller bio here.

Read Gio Andollo’s diary below to learn about how he finds and makes his meals, and how he gets by without a steady job.Editor’s note: Assume that all meals are not purchased unless otherwise mentioned.

Monday, July 18

9am: I roll out of bed finally.I observe Monday as Sabbath a day of rest, thanks, and realignment.A holy day.So I don’t have any work on the agenda.I do, however, have plenty planned for the day!

10am: Breakfast.Raisin bagel with peanut butter and jelly.

10:30am: I prepare a veggie stock with portobello, cucumber, broccoli stalk, parsnip, garlic.I head over to the credit union while the stock simmers to deposit my earnings from busking the past couple weeks.I love banking at my credit union in Washington Heights, as they are especially concerned about community development in my neighborhood.

1pm: Corn, tomato, potato are added to the stock for a soup that will last me at least 4 or 5 meals.I rescued all the aforementioned veggies last week on a trash tour near Columbia University

1:30pm: I catch up on last week’s news with Jon Stewart’s Daily Show, scarfing down my delicious freegan soup for lunch.

2pm: I’m headed to Brooklyn Boulders, a rock-climbing gym where I climb for free in exchange for weekly volunteer shifts.I make a stop on the way at the Union Square greenmarket where I’m dropping off a heavy wet bag full of last week’s compost (food waste and organic garbage).

6pm: I plan to meet and pray with friends at the 181 Street overlook in Washington Heights, but I fall asleep on the train as I make my way back home and miss my transfer.

9pm: Dinner.Homemade bagel pizzas with fresh mozzarella.I wash dishes while they bake, a chore so important to me that I’ve started a blog about it!My roommate complains about ants in the apartment, killing them mercilessly.I vouch for their right to life, citing their workmanship and sense of community.He’s not buying it.

12:20am: I’ve stayed up later than I should and for no good reason.Turn in.

Tuesday, July 19

8:10am: Breakfast.I’m down to my last bagel.Thankfully I have a trash tour scheduled for tonight.

8:30am: Begin recording new song.My studio is a workspace in one corner of the dining room.I lower all the windows and close the doors to keep it totally quiet.With the summer heat outside, this room is an oven.

6:23pm: Feeling very behind on my work but also exhausted from recording all day in this hot and musty office space.Don’t feel like going anywhere or doing anything.

7:10pm: Checking freegan.info e-mail inbox and updating @freegannyc on twitter.

9pm: Pantry dive (i.e.digging in the back of one’s pantrysuch food is typically long expired but often still viable and edible) for a rice pilaf and lentil meal that expired in 2003!I kid you not.But dry food doesn’t ever go bad does it?Only one way to find out!

9:55pm: I kill the stove and run out to meet a freegan cohort, Annie, for this week’s trash tour in Morningside Heights.Trash tours are at a pre-determined time and location, almost always in Morningside Heights around 10.We open trash bags on the curbside and rescue as much food as we can manage for one week’s consumption.Annie typically takes much more than she could handle alone, as she then shares much of the food with residents at her building.We typically put all the food on display as we dig, talking with pa*sersby, showing them the abundance of «waste» and food, and offering them the food also.Then we help ourselves to what’s left over, return whatever we don’t want to the garbage pile (always leaving any given location nicer than we found it!) and move on to the next grocery store.

11:40pm: Return with the booty: plenty of bread, fruits, veggies, herbs, eggrolls, veggie lo mein meals and a large block of blue cheese.I load it all into the fridge and freezer.

12am: Scarfing down the rice and lentils.

Wednesday, July 20

8:10am: Breakfast.PB&J bagel and a smoothie with apple, peach and pear.

9am: Back to recording!

11:30am: Pop a veggie lo mein in the microwave for the road.I eat half on the subway, headed to 33rd Street and Lexington Avenue to meet with a videographer colleague.I have some music video ideas to discuss with him.

1:30pm: Walk to 33rd and 5th to finish my lunch and meet with a pastor/mentor at the Trinity Grace Church offices.

3:05pm: I brought my guitar with me for these errands but left my picks at home.I don’t want to waste the ride back so I fashion guitar picks out of an old credit card and perform on the subway.

5:30pm: Head back out for a DIY show in Bed-Stuy Brooklyn.I busk all the way there, playing guitar, harmonica and singing popular covers.This is how I earn my living but I don’t earn much on this particular ride.As I walk the seven blocks to the apartment building where the rooftop show is to be held, I am robbed by a man who’s got at least a foot and a hundred pounds on me.He says it’s his girlfriend’s birthday and he wants to get her something nice.

6:42pm: I arrive at the show.I’m first on the bill so I take the stage around 7:15pm.There is a crowd of 30-40 people to see me play.The rest of the musical acts are varied and fun, the food is vegan and delicious, the weather is perfect and the overall vibe fantastic.I make a ton of new friends/fans and contacts as a tipping point nears.

12:25am: The show has ended.I leave alone but find some of my new friends on the subway platform.We sit and talk together.There is a ventriloquist on the train who does an impressive job cracking jokes with his puppet Suzy.

2am: I arrive at home, exhausted.I have to get some of these events off my chest via @giosafari on Twitter.

3:09am: Throw back some eggrolls and beer, lamenting how late it’s become.

Thursday, July 21

10am: I catch up on e-mail and prepare breakfast: oatmeal with dried cranberries and a smoothie with apple, pear and orange.

11:30am: Make plans for food rescue in the evening.

12:55pm: Everyone wants a revolution but nobody wants to do the dishes.

1:28pm: Lunch.Monday’s soup with bread roll.

3:40pm: I head up to Word Up Books, a new radical bookstore in Washington Heights, for my 4pm volunteer shift.There’s little traffic and not much to do so I pick up a book by Howard Zinn, Artists In Times of War.I’m inspired.

9:30pm: Watching a documentary about freegans in NYC.Many of my friends are featured and I make a short appearance myself.I should have went for a trash tour tonight for tomorrow’s party, but I didn’t have anyone to accompany me.I consider going alone but it’s getting too late.

10:53pm: Dinner.More eggrolls and an apple.

12:12am: I’ve stayed up a bit past schedule, watching the Daily Show and chatting with my roommates.Headed to bed!

Friday, July 22

9am: Finding open mics and couches to surf for next week’s Mini(apolis) Tour to Pittsburgh, Chicago, Cincinnati, Chicago and of course Minneapolis.

2:45pm: I begin tidying up the place for a DIY show I’m hosting at my apartment tonight.

3:45pm: Lunch.Homemade pizzas.

4:45pm: Help for tonight’s show begins to arrive.Freegan cohort Annie delivers a load of freegan eats for the party; the headliner from Florida (Marc of To All My Dear Friends) arrives and helps me set up a bit.Another friend comes and transforms Annie’s delivery into a freegan feast: pizza bites, fruit parfaits, chips and salsa, fruit punch and more!

7pm: Friends, fans, and performing artists trickle in, munch on the food, mingle.Cave Cricket, To All My Dear Friends, and Stefan Fink each play a set and I take the stage last around 11:30.This was probably the hottest day of the year and the little window AC and fans could hardly keep the space at a tolerable temperature.We made freegan popsicles from fresh fruit to keep folks cool and every one kept a real positive attitude throughout the night.

3:30am: I’ve stayed up real late talking with Marc about DIY music, booking, recording, touring, busking, etc.I take a cold shower and head to bed.

Saturday, July 23

7:30am: Alarm sounds.I get up and dressed, eat breakfast, grab my guitar and head out the door with Marc.I’m headed to Brooklyn Boulders again, but this time to work my volunteer shift.I busk all the way there.

9:41am: I’m 11 minutes late but nobody mentions it.I clock in and help stock the fridges with drinks.I’m on the clock for the next three hours.

11am: A children’s party begins and I’m charged with manning the climbing ropes as the kids ascend the walls.I enjoy teaching and working with kids, so this is definitely a fun time.Sure beats vacuuming the carpets and/or chalk-eater filters!

12:42pm: I’m off the clock.I walk over to a pizza place and buy a slicemy first food purchase all week.I eat and walk back to the gym and climb until around 2:30pm.Then head back home.

4pm: My apartment is sweltering.I try to work at my computer but can’t stay awake.I nap for about an hour.Then I get back to the computer and spend the rest of the night making plans for the tour next week and contacting local bands to book shows in NYC in August.I’m looking forward to putting on shows at the book store in Washington Heights!

11:30pm: Dinner.Some veggie lo mein and Monday night’s soup.An episode of the Daily Show.

Sunday, July 24

8:50am: I’m late.

9:08am: I arrive at PS 75, the venue for Trinity Grace Church’s Upper West Side morning service.I strap on my guitar, sound check and rehearse with the worship team until 10:45am.

11am: The service starts on time (this is rare but a rhythm we hope to establish.I clearly have much work to do on my own punctuality!).We hear a sermon on Psalm 37 sandwiched between two sets of worship songs.I love playing with this band of brothers and sisters!

1:28pm: I take to the subway, busking my way home.I don’t make much money and a group of men (in their early 20s, I think) ridicule me aloud while I play.As if this work wasn’t hard enough!

3pm: Lunch.Finishing yesterday’s veggie lo mein while continuing tour plans.

5:12pm: I get a text from a friend who is at the TGC Chelsea service, which started at 5pm.I’m coming to meet them after the service; I’m still at home and need to shower before I leave.I end up sleeping most of the subway ride to 23rd street.

6:30pm: The service is over and I’ve somehow missed my friend.I link up with other friends and go to a nearby restaurant.This is my one budgeted meal of the week and I’m allowed $10.I end up spending that and change.We all share good food and conversation.

10:45pm: Looking over my week, I’m disappointed in my lack of discipline going to bed and getting up each day.Nevertheless I’ve gotten much done and had a very productive week!

11:25pm: Dinner.Homemade pizzas and Monday’s soup, still going strong

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News: Reliance Controls TCA1006D Panel/Link 100-Amp Utility/60-Amp Generator Transfer Switch For Up To 15000-Watt Generators

News: Reliance Controls TCA1006D Panel/Link 100-Amp Utility/60-Amp  Generator Transfer Switch For Up To 15000-Watt Generators
Burt's Bees Honey &amp; Shea Butter Body Butter, 6.6-Ounce Jar Reviews

Thursday

Genome.gov | 2011 News Feature: NHGRI researchers identify gene for inherited bleeding disorder

Genome.gov | 2011 News Feature: NHGRI researchers identify gene for inherited bleeding disorder Associate Communications Director for Intramural ResearchLight microscopy of a peripheral blood smear of a patient with Gray platelet syndrome, left, shows pale gray platelets (arrows), some larger than normal.Normal, darkly stained platelets (arrows) are shown at right.An eight-year gene hunt has culminated successfully for National Human Genome Research Institute researchers studying the inherited bleeding disorder gray platelet syndrome (GPS).The researchers found that mutations in the NBEAL2 gene cause GPS.

The study, published in the July 17, 2011, early online issue of Nature Genetics, is the second publication from the group in the span of a year, cracking the DNA code for the condition after almost a decade of research and international collaboration to locate patients with the disorder.'Finding the GPS gene is an important step in improving our understanding of how certain organelles are formed,' said lead author Meral Gunay-Aygun, M.D., NHGRI staff clinician and an a*sociate professor in the Department of Pediatrics at Johns Hopkins Medicine in Baltimore.

Platelets have two major organelles-alpha-granules and delta-granules.Alpha-granules store proteins required for the formation of blood clots that stop bleeding.GPS is characterized by the absence of alpha-granules and their contents, which produces a bleeding disorder in patients with this condition.The syndrome derives its name from the gray appearance of platelets in light microscope images due to the lack of alpha-granules that give the platelets their normal purple color.

GPS is a progressive condition that is usually detected when patients experience bleeding and low platelet counts.The disorder also causes scar tissue in the bone marrow and enlargement of the spleen.Bleeding tendency in GPS is usually mild to moderate, but deaths have occurred due to hemorrhages, especially in advanced stages of the disease when platelet counts decrease even more.

Dr.Gunay-Aygun, lead investigator of the study, launched an NIH protocol in 2004 and recruited 26 GPS patients from 15 families around the world.The first attempts to locate the gene for GPS involved genetic linkage analysis of the DNA from patients recruited to NIH.The linkage analysis narrowed down the location of the GPS gene to a large region on chromosome 3 that was shared by the affected individuals from all families.Last year, the group published a paper in the journal Blood that reported the mapping results and described in detail the clinical features and natural history of the disease.

The 2010 study, based upon data from 25 patients in 14 families, detailed efforts to map the region including the GPS gene, which had been narrowed down to a region of 197 protein-coding genes on chromosome 3.In the current publication, the researchers zeroed in on the NBEAL2 gene through wh*le-exome sequencing, an approach that decodes the 1-2 percent of the genome that contains protein-coding genes.The sequencing and analysis was performed at the NIH Intramural Sequencing Center (NISC), administered by NHGRI.

'During the course of the project, we adapted our search to available technologies, getting progressively closer to finding the gene.Wh*le-exome sequencing enabled us to exclude a large number of genes and eventually identify NBEAL2 as the GPS gene.' Dr.Gunay-Aygun is a member of NHGRI's Medical Genetics Branch, where a key research focus is on disorders characterized by abnormalities in formation of organelle structures within cells.Impaired organelles, including lysosomes, which perform a housekeeping role within cells, can cause symptoms such as albinism, bleeding, lung damage and neurological problems depending on the particular disorder.

'Even though it is relatively rare, GPS is a well-known disorder among hematologists because the basic defect is so clear-cut,' Dr.Gunay-Aygun said about the disease first described in 1971.'Alpha-granules, the most abundant organelles in platelets are missing.What we learn about the function of NBEAL2 protein in platelets will probably help us understand how organelles form in other cells types as well.'

Despite early enthusiasm for finding the gene responsible for GPS-and long nights sequencing genes in the lab-Dr.Gunay-Aygun's expectations waivered.'This project seemed to run out of luck many times,' she said.But, according to Dr.Gunay-Aygun, perseverance paid off.

Initially the team sequenced more than 30 selected candidate genes in the region using traditional DNA sequencing methods but could not find any convincing mutations.Attempts to sequence all the genes in the region at NISC excluded 75 percent of the target region.Subsequent wh*le-exome sequencing did not identify mutations but significantly decreased the number of genes.

The researchers ultimately identified mutations in NBEAL2 by performing a final round of analysis of the remaining difficult-to-sequence genes.NBEAL2 is a large gene containing 52 exons.'It is not inviting to sequence it manually in the lab,' said co-author Marjan Huizing, Ph.D., NHGRI a*sociate investigator.Despite the difficulty, the researchers found mutations in each of the 15 GPS families in the study.'These are different mutations, but they all impair the function of the protein,' said Dr.Huizing.

The researchers have only begun to explore the function of the NBEAL2 protein and note that it is similar in composition to the protein defective in Chediak-Higashi syndrome, another organelle disorder.'The Chediak-Higashi syndrome gene was identified more than 10 years ago and its function is still not completely understood,' said Dr.Gunay-Aygun.'It sometimes takes a long time to determine the exact function of a protein.'

Dr.Gunay-Aygun hopes that the study of the underlying cell biological defect in GPS will contribute to our overall understanding of how organelles form and lead to new strategies for treatment of these disorders.

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THE STIRRING UP OF HATRED AGAINST JEWS, ISRAEL AND ZIONISM IS WHAT MUST BE FOUGHT IN NORWAY AND IN EUROPE

THE STIRRING UP OF HATRED AGAINST JEWS, ISRAEL AND ZIONISM IS WHAT MUST BE FOUGHT IN NORWAY AND IN EUROPE (Hungarian Jews arriving in Auschwitz in 1944 German Federal Archives) В  The essence of the political situation in Norway, especially in its foreign policy, is support for «Palestinians» and for «Palestinian State» В  Combined with hatred for Israel across the political
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The 411 on diets (Part 5) Blood type diet 101 - Manila Standard Today

The 411 on diets (Part 5) Blood type diet 101 - Manila Standard Today
The 411 connected diets (Part 5) bodily fluid type diet 101 manilla Standard Today Since bodily fluid never changes, following type A diet based on bodily fluid type also doesn't change.However, there are galore medical conditions that Gregorian calendar month develop as a someone ages such as diabetes, hypertension ( high bodily fluid pressure ), and intuition disease that may ask ... Link To Article

Voters blame Bush more than Obama for the economy

High blood pressure a hazard: blacks most at risk - Sowetan

High blood pressure a hazard: blacks most at risk - Sowetan
High blood force per unit area a hazard: blacks most at risk Sowetan i in every four southbound Africans between the ages of 15 and lxiv suffers from salt-sensitive advanced blood pressure - and this number is improving rapidly.Black South Africans appear to be more than at risk than others.A 2005 survey of nearly 10000 South Africans ... Link To Article

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All About Inexpensive Maternity Clothes Online

All About Inexpensive Maternity Clothes Online

Article by Kurt Ferguson

Maternity clothes online fall under specialty clothing as they are designed specifically for pregnant ladies.Therefore, these clothes are generally considered expensive.However, this is a myth because cheap clothes for mums-to-be can be found online.Pregnancy and rearing children can put a lot of financial burden on parents.On the top of that spending on clothes meant for pregnant women adds up to the cost.Pregnancy can bring many physical changes to a woman’s appearance.She needs clothes which can fit easily and are

Read More on Fregnancy .


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Causes and Cure to Vitamin B12 Deficiency

Causes and Cure to Vitamin B12 Deficiency

Johnson has reviewed the methods used to produce deficiency of B12 in pigs, chicks, and rats.On a "soy-protein synthetic milk" ration pigs develop B12 deficiency.Growth is poor and death ensues unless B12 is administered.Development offormed elements of blood is abnormal.The ease with which uncomplicated B12 deficiency can be produced in the young pig has made this animal a valuable tool in studying metabolic pathways involving the vitamin.Rats and chicks are susceptible to lack of dietary B12.В 
Growth is poor, and rats also develop porphyrin whiskers and scaly feet.Vitamin B12 relations to cobalt nutrition in ruminants are discussed with cobalt.In humans a dietary B12 deficiency is rare.In a review B12 deficiency symptoms of humans subsisting on diets devoid of animal products are discussed.Of more interest is the relation of the vitamin to human pernicious anemia and other diseases.Castle and others some years ago suggested that in pernicious anemia there is a deficiency of an intrinsic factor (stomach factor) and an extrinsic factor (food factor).The two factors were thought to react to form something required for the maturation of red blood cells.The extrinsic factor (EF) of Castle is now established to be vitamin B12.The intrinsic factor, a low-molecular-weight mucoprotein, normally occurs in gastric juice; and pernicious anemia is due to a lack of this substance, since B12 is not absorbed in its absence.The mechanism by which intrinsic factor brings about absorption is still not clear.However, it has been proposed that IF removes the vitamin from natural protein complexes with animal proteins.It also brings about absorption of the vitamin into the mucosal cells with the aid of an intestinal juice factor called releasing factor.В 
Herbert studied the source of this factor in rats and found it to be in the proximal end of the small intestine.It is known that IF has a high degree of species specificity.Fortunately hog and human IF have similar actions in humans.In very large doses B12 is absorbed in humans without IF, but not with doses found in ordinary diets.Small doses parenterally are highly effective in deficiency states.After absorption into the blood, B12 is bound to plasma proteins and may circulate to the sites of activity.That portion converted into coenzymes is stored principally in the liver.Small amounts of B12 occur in blood of normal individuals.В 
The variations are wide, but around 100 micro-g everal hundred mg per ml of blood have been found and a tenth or less of these amounts in pernicious anemia patients.The stools of pernicious anemia patients contain large amounts of the vitamin after oral administration if no intrinsic factor is given.A highly potent intrinsic factor preparation from hog pyloric mucosa was clinically active at a level of 0.3 mg; that is, it increased B12 absorption in pernicious anemia patients at this level.The molecular weight was found to be about 5000; the material contained 10 per cent nitrogen and less than 3 per cent glucosamine.The binding capacity in vitro was found to be 3 ~g of B12 per mg.A comprehensive review on all aspects of intrinsic factor appeared in 1963 and a shorter review was published the same year.Another role of intrinsic factor appears to involve retention of B12 by tissues, Active preparations increased B12 uptake ofliver slices.В 
In liver perfusion experiments B12-C060 was taken up to a slight extent, and the uptake was tremendously enhanced in the presence of intrinsic factor.Sorbitol (sugar alcohol analogue of glucose) enhances B12 absorption in man.In rats this compound, manuitol, sorbose, and xylose also increased absorption.In normal persons, studies with C060-vitamin B12 show that over 70 per cent of a 0.5 ~g dose is absorbed and that increasing the dose results in lower percentage absorption.With a 5.0 j.Jg dose only 30 per cent was absorbed.Vitamin B12 is used clinically in diseases other than pernicious anemia.It is effective in various megaloblastic anemias and in neurological disturbances accompanying various other conditions.Much of the information in this connection has been brought together and summarized.В 

Stem Cell Breakthrough Heralds New Era Of Therapy Development

Stem Cell Breakthrough Heralds New Era Of Therapy Development
Scientists from the universities of Southampton and Glasgow have uncovered a new method for culturing adult stem cells which could lead to the creation of revolutionary stem cell therapies for conditions such as arthritis, Alzheimer's disease and Parkinson's disease.

The research, funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the University of Glasgow and published in the journal Nature Materials, shows how a new nanoscale plastic can cheaply and easily solve a problem which has previously made the expansion of stem cells for therapeutic purposes impossible.

Currently, when adult stem cells are harvested from a patient, they are cultured in the laboratory to increase the initial yield of cells and create a batch of sufficient volume to kick-start the process of cellular regeneration when they are reintroduced back into the patient.

The process of culturing is made more difficult by spontaneous stem cell differentiation, where stem cells grown on standard plastic tissue culture surfaces do not expand to create new stem cells but instead create other cells which are of no use in therapy.Currently, stem cell expansion is often boosted by immersing the cells in chemical solutions which help to increase the overall yield of stem cells but are limited in their effectiveness.

The new nanopatterned surface, developed and fabricated at the University of Glasgow, is designed to offer a method of stem cell expansion which is much easier to manufacture and use than anything currently available.Created by an injection-moulding process similar to that which is used to manufacture Blu-ray discs, the surface is covered with 120-nanometre pits which the researchers have found is much more effective in allowing stem cells to grow and spread whilst retaining their stem cell characteristics.

Dr Matthew Dalby from the University of Glasgow, who led the research alongside colleagues Dr Nikolaj Gadegaard and Professor Richard Oreffo of the University of Southampton, explains: «Until now, it’s been very difficult to grow stem cells in sufficient numbers and maintain them as stem cells for use in therapy.What we and our colleagues at the University of Southampton have shown is that this new nanostructured surface can be used to very effectively culture mesencyhmal stem cells, taken from sources such as bone marrow, which can then be put to use in musculoskeletal, orthopaedic and connective tissues.».

«If the same process can be used to culture other types of stem cells too, and this research in under way in our labs, our technology could be the first step on the road to developing large-scale stem cell culture factories which would allow for the creation of a wide range of therapies for many common diseases such as diabetes, arthritis, Alzheimer's disease and Parkinson's disease.We’re very excited about the potential applications of the technology and we’re already in the early stages of conversations to make the surface commercially available.»

Professor Richard Oreffo, who led the University of Southampton team, adds: «Development of platform technologies that allow the scale up of skeletal or mesenchymal stem cells offers a wh*le new approach to skeletal regenerative medicine.If this new technology enables us to create sufficient stem cells, and to pattern hip implants, for example, it could herald the development of new medical devices with therapeutic application and approaches to understanding stem cell fate and regulation.

«It is important to realise the ability to retain skeletal stem cell phenotype using surface topography offers a step change in current approaches for stem cell biology.The implications for research and future interventions for patients with arthritis and other musculoskeletal diseases are substantial.»

Professor Douglas Kell, Chief Executive, BBSRC says: «Understanding how stem cells are affected by their environment is key to appreciating how they might be grown in sufficient quantities to be used in research or as therapies.This research shows that the physical surface that the cells are grown on can actually affect their fundamental biology in ways that are useful for us.

«Multidisciplinary research is increasingly important and this project is a great example where cell biology, medicine, and engineering come together in powerful synergy to solve a complex problem.»

The paper, titled в

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Culture Media For bacteria - Types Of culture Media and Some Important Culture Media

Monday

Evolution of Language Parallels Evolution of Species | Wired Science | Wired.com

Evolution of Language Parallels Evolution of Species | Wired Science | Wired.com

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Mitch McConnell: Grand Bargain On Debt Limit Unlikely

MAN STEALS AMBULANCE AT ACCIDENT SCENE

MAN STEALS AMBULANCE AT ACCIDENT SCENE

Police said Travis Ward, 28, boosted the empty emergency vehicle around 4 a.m.Sunday while nearly a dozen cops and firefighters battled to save lives from a raging blaze at a home in west Phoenix.

Rescue crews had left the engine running in order to keep the vehicle cool for any burn victims they managed to pull from the blaze, Phoenix police spokesman Sgt.Tommy Thompson told the Arizona Republic.

Ward drove for several blocks, tearing up people’s lawns and smashing into a stop sign, a brick wall and several parked cars, cops said.

The terrifying joyride came to an end after Ward plowed into a house a few blocks from the fire, officials said.

No one was hurt in early morning mayhem, cops said.

Ward was arrested and charged with theft and criminal damage.Cops said he admitted to smoking marijuana before the crash-course caper.

Two people inside the home got out safely, and one was treated for smoke inhalation, officials said.

Fire Capt.Troy Caskey said the ambulance, Rescue 26, was «wrecked» and could be headed for the junk heap, the Republic reported.


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A Look at &lt;i&gt;Kandireega&lt;i&gt;

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News: Xbox 360 Wireless Microphone

Sunday

'High BP, tobacco use lead to a stroke' - Daily News &amp; Analysis

'High BP, tobacco use lead to a stroke' - Daily News &amp; Analysis
' High BP, tobacco use lead to a stroke' Daily tidings & Analysis High bodily fluid pressure , use of tobacco and lack of physical activity are the main factors which could lead to a maneuver according to Dr NR Ichaporia, head of the department of the Jehangir Hospital.Dr Ichaporia was delivering a lecture connected 'preventing and ... Link To Article

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